1. NGS.git
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  3. NGS
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  MooX added ability to set max x & y-axis values in chartdirector output; 02-vcf-analysis script loads ngs.locations db table for use in start position exclusions 11 years ago
  Test moved NGS::Test function to NGS::Test::Common & moved test-lib.pl functions to NGS::Test, removed all calls to test-lib.pl from tests 11 years ago
  VEP re-arranged order of loaded modules in NGS.pm to avoid redefined var warning; moved vep results from template var to vars var; replace BioSphere::api_version with a Moo 'has' api_version to avoid error in get_cache_types() when called from NGS.pm 11 years ago
  ChartMaker.pm 2kB added ability to set max x & y-axis values in chartdirector output; 02-vcf-analysis script loads ngs.locations db table for use in start position exclusions 11 years ago
  DB.pm 539B replaced Dancer2::FileUtils with Path::Tiny for path() method 11 years ago
  Test.pm 3kB moved NGS::Test function to NGS::Test::Common & moved test-lib.pl functions to NGS::Test, removed all calls to test-lib.pl from tests 11 years ago
  VEP.pm 35kB major rewrite of test scripts - adapted for D2 version .154 - replaced D2::Test with Plack::Test; new NGS::Test Import::Base class 11 years ago
  README.txt
http://www.ensembl.org/info/docs/variation/vep/vep_script.html Setup/update VEP - delete scripts/variant_effect_predictor dir extract variant_effect_predictor.tar.gz into scripts dir run scripts/variant_effect_predictor/INSTALL.pl - skip cache. Cache manually installed/updated (needs to match VEP version) from: ftp://ftp.ensembl.org/pub/release-<xx>/variation/VEP/ see README.txt in NGS/script dir for more details Cache directory structure: vep dir containing core & refseq dirs, each with homo_sapiens/xx cache * on dev box - symlink /home/raj/.vep -> /media/sf_WIN_DRIVE/vep * on deployment - /home/raj/.vep to run from command-line: perl script/variant_effect_predictor.pl -config=script/vep.ini \ -i=t/data/myeloid_variants.vep -o=output.txt --polyphen=b --sift=b \ --check_existing --coding_only --regulatory --dir=refseq/core or: perl script/variant_effect_predictor.pl -config=/home/raj/apps/NGS/script/vep.ini \ -o=output.txt --dir=/home/raj/.vep/refseq --polyphen=b --sift=b --check_existing \ --coding_only --regulatory -i=/tmp/20_06_14.vep PERFORMANCE: a) 4055-row vep input with unsorted chromosome order on 163.160.171.48: fork=0 255 sec fork=2 580 sec fork=4 620 sec b) 1028-row vep input with alpha-numerically sorted chromosome order on 163.160.171.48: fork=0 95 sec fork=2 65 sec (optimal) fork=3 68 sec fork=4 70 sec fork=5 75 sec fork=6 75 sec performance much WORSE on dev server with fork enabled splitting a 1110-row vep input into separate chromosome input files slightly REDUCED performance (77sec vs 66sec) on dev