# no_stats is essential if running through piped fh, or while loop never exits; # port only used for offline mode (ensembl site); fork does not give sig. speed # boost (16 sec for 880 row input with fork = 0 and fork = 4); fork _does_ # disrupt sort order of results; fork maybe causing script to hang occasionally; # cache + offline uses local resource (/home/raj/.vep); force_overwrite required # if output file already exists; sift, polyphen, check_existing, coding_only & # regulatory params supplied from web form #fork 2 # worse for dev - selectively enabled in VEP.pm #core_type core # can't find documentation for this opt cache 1 dir /home/raj/.vep force_overwrite 1 host ensembldb.ensembl.org no_stats 1 offline 1 output_file stdout port 5306 species homo_sapiens # needs full path to fasta file - vep.pl does: "die [...] unless -e $config->{fasta}" fasta /home/raj/.vep/fasta/Homo_sapiens.GRCh37.75.dna.primary_assembly.fa