# use Modern::Perl qw(2012); # not supported in perl 5.10
# use autodie;
# TODO: exons not sorted naturally; data supplied to tt as chr:location key, not exon
package NGS;
use Dancer2 ':syntax';
use Dancer2::Core::Error;
# use Dancer::Plugin::Database; # doesn't work in D2
use Time::HiRes qw(gettimeofday tv_interval);
use Data::Dump qw(dump);
use Carp qw(croak);
use Template;
use IO::All;
use NGS::VEP;
our $VERSION = '0.1';
# --- Globals ------------------------------------------------------------------
use constant VEP_SRC_FILENAME => 'data_src'; # form param containing filename
use constant VEP_INPUT_TT => 'vep.tt'; # tmpl contains vep data input form
use constant VEP_OUTPUT_TT => 'vep/result.tt'; # tmpl to handle vep results
#-------------------------------------------------------------------------------
hook before_template_render => sub {
my $tokens = shift;
$tokens->{nsort_labno} = sub {
my $list = shift || return; # warn dump $list;
return sort _sort_by_labno(@$list);
};
$tokens->{nsort_exon} = sub { # can't do this yet - key = chr:location not exon
my $list = shift || return; # warn dump $list;
return sort _sort_by_exon(@$list);
};
};
#-------------------------------------------------------------------------------
get '/' => sub { template 'index' }; # default index page
#-------------------------------------------------------------------------------
get '/vep' => sub {
debug 'GET upload page';
template VEP_INPUT_TT, { title => 'Upload VEP DataFile' };
};
#-------------------------------------------------------------------------------
post '/vep' => sub {
debug 'POST upload page'; # debug "Params are : " . dump( request->params );
my %allparams = params; # debug dump \%allparams;
my $input = {
data_file => upload(VEP_SRC_FILENAME) || undef,
form_opts => \%allparams,
};
_validate_user_input($input); debug 'Validation results: ' . dump($input);
#-- display any errors
if ( exists $input->{upload_error} ) {
debug 'have upload error';
return template VEP_INPUT_TT, {
title => 'Upload Data Error',
warning_message => 'You must upload the file again!',
%$input,
};
}
#-- passed validation
debug 'OK passed the error checks.';
my $src = $input->{data_file}->content; # debug $data;
my $opts = $input->{form_opts};
# add env for debug rules:
# $opts->{env} = request->env; # can't find way of using it
my $t0 = [gettimeofday]; # set t0
# send submitted src data to vep script:
my $data = _parse({ src => $src, opts => $opts });
$data->{runtime} = tv_interval $t0, [gettimeofday]; # vep script runtime
# delete temp file (if exists):
my $tmp_file = $input->{data_file}->tempname;
io($tmp_file)->unlink if -e $tmp_file;
return template VEP_OUTPUT_TT, { results => $data };
};
#-------------------------------------------------------------------------------
sub _parse {
my $args = shift;
my %args = (
form_opts => $args->{opts},
src_data => $args->{src},
);
my $parser = NGS::VEP->new(%args);
return $parser->result;
}
#-------------------------------------------------------------------------------
sub _validate_user_input {
my $validation_report = shift;
if ( defined $validation_report->{data_file} ) {
debug 'At least there is a file uploaded.';
#------ Check that the file is a valid data file
$validation_report->{upload_error} = 'Not a valid data file!'
unless _validate_file( $validation_report->{data_file} );
}
else {
$validation_report->{upload_error} = 'No data file uploaded!';
}
}
#-------------------------------------------------------------------------------
sub _validate_file {
my $in_file = shift; # warn $in_file;
my $cfg = config->{InputFile};
my $max_file_size = $cfg->{max_file_size} * 1024 * 1024; # MB
my $file_suffix = $cfg->{file_suffix};
return (
$in_file
&& ( lc $in_file->basename =~ /$file_suffix\Z/ )
&& ( $in_file->size <= $max_file_size )
);
}
#-------------------------------------------------------------------------------
sub _sort_by_labno {
my ($y1, $r1) = split '_', $a; # warn Dumper [$r1, $y1];
my ($y2, $r2) = split '_', $b; # warn Dumper [$r2, $y2];
return $y1 <=> $y2 || $r1 <=> $r2;
}
#-------------------------------------------------------------------------------
sub _sort_by_exon {
return $a cmp $b;
}
true;